Question 3#

Characteristics of tumorigenic transformation of cells include which of the following?

a. Enhanced surface adherence
b. Monolayer confluence inhibition
c. Acquisition of chemo-resistance
d. Immortalization

Correct Answer is D


Abnormally proliferating, transformed cells outgrow normal cells in the culture dish (ie, in vitro) and commonly display several abnormal characteristics. These include loss of contact inhibition (ie, cells continue to proliferate after a confluent monolayer is formed); an altered appearance and poor adherence to other cells or to the substratum; loss of anchorage dependence for growth; immortalization; and gain of tumorigenicity (ie, the ability to give rise to tumors when injected into an appropriate host).